Article
Detection of low-abundance point mutations by competitive strand assisted endonuclease IV signal amplification system.
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban - 1 Oct 2017
Xiong Fei, Liu Chuan-Zhen, Li Wang-Qiang, Dong Zi-Qiang, Zhang Jie
Abstract excerpt
Genetic mutations are important molecular biomarkers for cancer diagnosis and surveillance. Therefore, the development of methods for mutation detection characterized with straightforward, highly specific and sensitive to low-level mutations within various sequence contexts is extremely needed. Although some of the currently available methods have shown very encouraging results, their discrimination efficiency is...
Topics
- DNA
- Deoxyribonuclease IV (Phage T4-Induced)
- Fluorescent Dyes
- Genetic Predisposition to Disease
- Humans
- Neoplasms
- Point Mutation
- Real-Time Polymerase Chain Reaction
- Transition Temperature