Back to search

Article

Multiplexed Isothermal Nucleic Acid Detection Using Sequence-Specific Cleavage Mediated by Repair Endonucleases

2026-06-02

Abstract excerpt

<h4>ABSTRACT</h4> Rapid, portable detection of multiple nucleic acid targets is essential for infectious disease surveillance and precision oncology. CRISPR-based diagnostics have set a high bar for sensitivity and single-base specificity, yet their reliance on collateral nuclease activity complicates multiplexing, integration with amplification, and point-of-care deployment. Here we present TIMBER (Templated Inc...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work
889070dd-2c6f-5915-a1f0-42ac2a02e8b8
DOI
10.64898/2026.05.29.26354475
Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest
Interactive article relationship graphSelect a related publication card to move it into the centre and load its closest explainable connections. Solid lines are source-backed structured connections. Dashed lines are semantic discovery signals and are not scientific evidence.
Multiplexed Isothermal Nucleic Acid Detection Using Sequence-Specific Cleavage Mediated by Repair EndonucleasesDOI 10.64898/2026.05.29.26354475
One-Pot Isothermal Linear Amplification and Cas12a-based Nucleic Acid DetectionSemantic proximity
One-pot Point-of-Care Nucleic-acid Detection via Padlock DNA Ligation and Rolling Circle TranscriptionSemantic proximity
Enhanced SfaTnpB enables single-base-specific, one-pot nucleic acid detection for high-sensitivity diagnostics.Semantic proximity
Accelerated RNA detection using tandem CRISPR nucleasesSemantic proximity
Recent advances in isothermal amplification techniques coupled with clustered regularly interspaced short palindromic repeat/Cas systemsSemantic proximity
HOLMESv2: A CRISPR-Cas12b-Assisted Platform for Nucleic Acid Detection and DNA Methylation QuantitationSemantic proximity
Rapid, point-of-care molecular diagnostics with Cas13Semantic proximity
RAPID: Evaluation of Cas12a Protospacer Nicking and Chimeric Reporters for PAM-independent RNA and DNA diagnosticsSemantic proximity
STAR-CRISPR: a one-pot ultraspecific CRISPR strategy for rapid, visualized SNV detection and genotyping in point-of-care diagnostics.Semantic proximity
One-Pot Endonucleolytically Exponentiated Rolling Circle Amplification by CRISPR-Cas12a Affords Sensitive, Expedited Isothermal Detection of MicroRNAsSemantic proximity
A general strategy for highly sensitive analysis of genetic biomarkers at single-base resolution with ligase-based isothermally exponential amplification.Semantic proximity
Virus detection via programmable Type III-A CRISPR-Cas systemsSemantic proximity
Select a neighboring publication to make it the new centre.