Article
Generation of a multipurpose Prdm16 mouse allele by targeted gene trapping.
Disease models & mechanisms - 1 Jul 2017
Strassman Alexander, Schnütgen Frank, Dai Qi, Jones Jennifer C, Gomez Angela C, Pitstick Lenore, Holton Nathan E, Moskal Russell, Leslie Erin R, von Melchner Harald, Beier David R, Bjork Bryan C
Abstract excerpt
Gene trap mutagenesis is a powerful tool to create loss-of-function mutations in mice and other model organisms. Modifications of traditional gene trap cassettes, including addition of conditional features in the form of Flip-excision (FlEx) arrays to enable directional gene trap cassette inversions by Cre and Flpe site-specific recombinases, greatly enhanced their experimental potential. By taking advantage of...
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