Article
LoxP-FRT Trap (LOFT): a simple and flexible system for conventional and reversible gene targeting.
BMC biology - 30 Nov 2012
Chaiyachati Barbara H, Kaundal Ravinder K, Zhao Jiugang, Wu Jie, Flavell Richard, Chi Tian
Abstract excerpt
BACKGROUND: Conditional gene knockout (cKO) mediated by the Cre/LoxP system is indispensable for exploring gene functions in mice. However, a major limitation of this method is that gene KO is not reversible. A number of methods have been developed to overcome this, but each method has its own limitations. RESULTS: We describe a simple method we have named LOFT [LoxP-flippase (FLP) recognition target (FRT) Trap],...
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