Article
Genomewide production of multipurpose alleles for the functional analysis of the mouse genome.
Proceedings of the National Academy of Sciences of the United States of America - 17 May 2005
Schnütgen Frank, De-Zolt Silke, Van Sloun Petra, Hollatz Melanie, Floss Thomas, Hansen Jens, Altschmied Joachim, Seisenberger Claudia, Ghyselinck Norbert B, Ruiz Patricia, Chambon Pierre, Wurst Wolfgang, von Melchner Harald
Abstract excerpt
A type of retroviral gene trap vectors has been developed that can induce conditional mutations in most genes expressed in mouse embryonic stem (ES) cells. The vectors rely on directional site-specific recombination systems that can repair and re-induce gene trap mutations when activated in succession. After the gene traps are inserted into the mouse genome, genetic mutations can be produced at a particular time...
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