Article
Generation of a series of knock-in alleles using RMCE in ES cells.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2011
Roebroek Anton J M, Gordts Philip L S M, Reekmans Sara
Abstract excerpt
Recombinase-mediated cassette exchange (RMCE) is a powerful tool to generate a series of knock-in mutations into a particular gene of the mouse. It uses standard ES cell technologies to introduce an exchangeable cassette into the gene of interest by homologous recombination. Because the introduced exchangeable cassette is flanked by heterotypic specific recombination target sequences, site-specific recombinases...
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