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Article

Dual RMCE for efficient re-engineering of mouse mutant alleles.

Nature methods - 1 Nov 2010

Osterwalder Marco, Galli Antonella, Rosen Barry, Skarnes William C, Zeller Rolf, Lopez-Rios Javier

Abstract excerpt

We have developed dual recombinase-mediated cassette exchange (dRMCE) to efficiently re-engineer the thousands of available conditional alleles in mouse embryonic stem cells. dRMCE takes advantage of the wild-type loxP and FRT sites present in these conditional alleles and in many gene-trap lines. dRMCE is a scalable, flexible tool to introduce tags, reporters and mutant coding regions into an endogenous locus of...

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Identifiers

PMID: 20953177
DOI: 10.1038/nmeth.1521
PMCID: PMC3576631

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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