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Article

Floxin, a resource for genetically engineering mouse ESCs.

Nature methods - 1 Jan 2010

Singla Veena, Hunkapiller Julie, Santos Nicole, Seol Allen D, Norman Andrew R, Wakenight Paul, Skarnes William C, Reiter Jeremy F

Abstract excerpt

We describe a method for the highly efficient and precise targeted modification of gene trap loci in mouse embryonic stem cells (ESCs). Through the Floxin method, gene trap mutations were reverted and new DNA sequences inserted using Cre recombinase and a shuttle vector, pFloxin. Floxin technolog...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 19966808
DOI: 10.1038/nmeth.1406
PMCID: PMC2895430

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-19966808-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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