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Article

Interrogation of Functional miRNA-Target Interactions by CRISPR/Cas9 Genome Engineering.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017

Michaels Yale S, Wu Qianxin, Fulga Tudor A

Abstract excerpt

Post-transcriptional silencing by microRNAs (miRNAs) is a critical constituent of eukaryotic gene regulation. miRNAs are short (~22nt) noncoding RNAs capable of specifically targeting the miRNA-induced-silencing-complex (miRISC) to transcripts bearing a complementary miRNA response element (MRE). Although recent methodological advances have greatly improved our understanding of miRNA biogenesis and the mechanisms...

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PMID: 28439828
DOI: 10.1007/978-1-4939-6866-4_7

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

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