Article
CRISPR screening strategies for microRNA target identification.
The FEBS journal - 1 Jul 2020
Yang Bing, McJunkin Katherine
Abstract excerpt
Identifying microRNA (miRNA) target genes remains a major challenge in understanding the roles miRNAs play in gene regulation. Furthermore, understanding which miRNA-target interactions are the most biologically important is even more difficult. We present CRISPR-based strategies to identify essential miRNA binding sites. First, CRISPR knockout screens can easily be adapted to identify genes whose inactivation...
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