Genetic Modification in Human Pluripotent Stem Cells by Homologous Recombination and CRISPR/Cas9 System.

doi:10.1007/7651_2014_73

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Article

Genetic Modification in Human Pluripotent Stem Cells by Homologous Recombination and CRISPR/Cas9 System.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2016

Xue Haipeng, Wu Jianbo, Li Shenglan, Rao Mahendra S, Liu Ying

Abstract excerpt

Genetic modification is an indispensable tool to study gene function in normal development and disease. The recent breakthrough of creating human induced pluripotent stem cells (iPSCs) by defined factors (Takahashi et al., Cell 131:861-872, 2007) provides a renewable source of patient autologous cells that not only retain identical genetic information but also give rise to many cell types of the body including...

Topics

Animals
Blotting, Southern
CRISPR-Cas Systems
Cell Survival
Electroporation
Fibroblasts
Gene Targeting
Genes, Reporter
Genetic Engineering
Genetic Vectors
Homologous Recombination

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Humans

Mice

Mutation

Pluripotent Stem Cells

RNA, Guide, CRISPR-Cas Systems

SOXB1 Transcription Factors

Transfection

Source

Open in PubMed

Identifiers

PMID: 24615461
DOI: 10.1007/7651_2014_73

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-24615461-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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