Article
A highly precise and portable genome engineering method allows comparison of mutational effects across bacterial species.
Proceedings of the National Academy of Sciences of the United States of America - 1 Mar 2016
Nyerges Ákos, Csörgő Bálint, Nagy István, Bálint Balázs, Bihari Péter, Lázár Viktória, Apjok Gábor, Umenhoffer Kinga, Bogos Balázs, Pósfai György, Pál Csaba
Abstract excerpt
Currently available tools for multiplex bacterial genome engineering are optimized for a few laboratory model strains, demand extensive prior modification of the host strain, and lead to the accumulation of numerous off-target modifications. Building on prior development of multiplex automated genome engineering (MAGE), our work addresses these problems in a single framework. Using a dominant-negative mutant...
Topics
- Bacteria
- Genetic Engineering
- Genome, Bacterial
- Mutation