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Article

Multiplex genome editing by natural transformation.

Proceedings of the National Academy of Sciences of the United States of America - 17 Jun 2014

Dalia Ankur B, McDonough EmilyKate, Camilli Andrew

Abstract excerpt

Editing bacterial genomes is an essential tool in research and synthetic biology applications. Here, we describe multiplex genome editing by natural transformation (MuGENT), a method for accelerated evolution based on the cotransformation of unlinked genetic markers in naturally competent microorganisms. We found that natural cotransformation allows scarless genome editing at unprecedented frequencies of ∼50%....

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 24889608
DOI: 10.1073/pnas.1406478111
PMCID: PMC4066482

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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