Article
Transient overexpression of DNA adenine methylase enables efficient and mobile genome engineering with reduced off-target effects.
Nucleic acids research - 29 Feb 2016
Lennen Rebecca M, Nilsson Wallin Annika I, Pedersen Margit, Bonde Mads, Luo Hao, Herrgård Markus J, Sommer Morten O A
Abstract excerpt
Homologous recombination of single-stranded oligonucleotides is a highly efficient process for introducing precise mutations into the genome of E. coli and other organisms when mismatch repair (MMR) is disabled. This can result in the rapid accumulation of off-target mutations that can mask desired phenotypes, especially when selections need to be employed following the generation of combinatorial libraries....
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