Rapid profiling of a microbial genome using mixtures of barcoded oligonucleotides.

doi:10.1038/nbt.1653

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Article

Rapid profiling of a microbial genome using mixtures of barcoded oligonucleotides.

Nature biotechnology - 1 Aug 2010

Warner Joseph R, Reeder Philippa J, Karimpour-Fard Anis, Woodruff Lauren B A, Gill Ryan T

Abstract excerpt

A fundamental goal in biotechnology and biology is the development of approaches to better understand the genetic basis of traits. Here we report a versatile method, trackable multiplex recombineering (TRMR), whereby thousands of specific genetic modifications are created and evaluated simultaneously. To demonstrate TRMR, in a single day we modified the expression of >95% of the genes in Escherichia coli by...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 20639866
DOI: 10.1038/nbt.1653

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.