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Article

Trackable multiplex recombineering for gene-trait mapping in E. coli.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2013

Mansell Thomas J, Warner Joseph R, Gill Ryan T

Abstract excerpt

Recent advances in homologous recombination in Escherichia coli have enabled improved genome engineering by multiplex recombineering. In this chapter, we present trackable multiplex recombineering (TRMR), a method for gene-trait mapping which creates simulated knockdown and overexpression mutants for virtually all genes in the E. coli genome. The method combines oligonucleotide synthesis with multiplex...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 23417807
DOI: 10.1007/978-1-62703-299-5_12

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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