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Article

DNA gap repair in Escherichia coli for multiplex site-directed mutagenesis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology - 1 May 2020

Lyozin George T, Brunelli Luca

Abstract excerpt

Site-directed mutagenesis allows the generation of novel DNA sequences that can be used for a variety of important applications such as the functional analysis of genetic variants. To overcome the limitations of existing site-directed mutagenesis approaches, we explored in vivo DNA gap repair. We found that site-specific mutations in plasmid DNA can be generated in Escherichia coli using mutant single-stranded...

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PMID: 32167210
DOI: 10.1096/fj.201902260R

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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