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Article

Genome-wide mapping of mutations at single-nucleotide resolution for protein, metabolic and genome engineering.

Nature biotechnology - 1 Jan 2017

Garst Andrew D, Bassalo Marcelo C, Pines Gur, Lynch Sean A, Halweg-Edwards Andrea L, Liu Rongming, Liang Liya, Wang Zhiwen, Zeitoun Ramsey, Alexander William G, Gill Ryan T

Abstract excerpt

Improvements in DNA synthesis and sequencing have underpinned comprehensive assessment of gene function in bacteria and eukaryotes. Genome-wide analyses require high-throughput methods to generate mutations and analyze their phenotypes, but approaches to date have been unable to efficiently link the effects of mutations in coding regions or promoter elements in a highly parallel fashion. We report that...

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PMID: 27941803
DOI: 10.1038/nbt.3718

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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