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Article

Rapid editing and evolution of bacterial genomes using libraries of synthetic DNA.

Nature protocols - 1 Oct 2014

Gallagher Ryan R, Li Zhe, Lewis Aaron O, Isaacs Farren J

Abstract excerpt

Multiplex automated genome engineering (MAGE) is a powerful technology for in vivo genome editing that uses synthetic single-stranded DNA (ssDNA) to introduce targeted modifications directly into the Escherichia coli chromosome. MAGE is a cyclical process that involves transformation of ssDNA (by electroporation) followed by outgrowth, during which bacteriophage homologous recombination proteins mediate annealing...

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PMID: 25188632
DOI: 10.1038/nprot.2014.082

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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