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Article

Conditional DNA repair mutants enable highly precise genome engineering.

Nucleic acids research - 1 Apr 2014

Nyerges Ákos, Csorgő Bálint, Nagy István, Latinovics Dóra, Szamecz Béla, Pósfai György, Pál Csaba

Abstract excerpt

Oligonucleotide-mediated multiplex genome engineering is an important tool for bacterial genome editing. The efficient application of this technique requires the inactivation of the endogenous methyl-directed mismatch repair system that in turn leads to a drastically elevated genomic mutation rate and the consequent accumulation of undesired off-target mutations. Here, we present a novel strategy for mismatch...

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Identifiers

PMID: 24500200
DOI: 10.1093/nar/gku105
PMCID: PMC4005651

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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