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Article

High-throughput functional variant screens via in vivo production of single-stranded DNA.

Proceedings of the National Academy of Sciences of the United States of America - 4 May 2021

Schubert Max G, Goodman Daniel B, Wannier Timothy M, Kaur Divjot, Farzadfard Fahim, Lu Timothy K, Shipman Seth L, Church George M

Abstract excerpt

Creating and characterizing individual genetic variants remains limited in scale, compared to the tremendous variation both existing in nature and envisioned by genome engineers. Here we introduce retron library recombineering (RLR), a methodology for high-throughput functional screens that surpasses the scale and specificity of CRISPR-Cas methods. We use the targeted reverse-transcription activity of retrons to...

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Identifiers

PMID: 33906944
DOI: 10.1073/pnas.2018181118
PMCID: PMC8106316

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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