Article
Generation of gene-modified goats targeting MSTN and FGF5 via zygote injection of CRISPR/Cas9 system.
Scientific reports - 10 Sept 2015
Wang Xiaolong, Yu Honghao, Lei Anmin, Zhou Jiankui, Zeng Wenxian, Zhu Haijing, Dong Zhiming, Niu Yiyuan, Shi Bingbo, Cai Bei, Liu Jinwang, Huang Shuai, Yan Hailong, Zhao Xiaoe, Zhou Guangxian, He Xiaoling, Chen Xiaoxu, Yang Yuxin, Jiang Yu, Shi Lei, Tian Xiue, Wang Yongjun, Ma Baohua, Huang Xingxu, Qu Lei, Chen Yulin
Abstract excerpt
Recent advances in the study of the CRISPR/Cas9 system have provided a precise and versatile approach for genome editing in various species. However, the applicability and efficiency of this method in large animal models, such as the goat, have not been extensively studied. Here, by co-injection of one-cell stage embryos with Cas9 mRNA and sgRNAs targeting two functional genes (MSTN and FGF5), we successfully...
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