Article
Efficient gene knockout in goats using CRISPR/Cas9 system.
PloS one - 1 Jan 2014
Ni Wei, Qiao Jun, Hu Shengwei, Zhao Xinxia, Regouski Misha, Yang Min, Polejaeva Irina A, Chen Chuangfu
Abstract excerpt
The CRISPR/Cas9 system has been adapted as an efficient genome editing tool in laboratory animals such as mice, rats, zebrafish and pigs. Here, we report that CRISPR/Cas9 mediated approach can efficiently induce monoallelic and biallelic gene knockout in goat primary fibroblasts. Four genes were disrupted simultaneously in goat fibroblasts by CRISPR/Cas9-mediated genome editing. The single-gene knockout...
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