Article
Use of CRISPR/Cas9 technology efficiently targetted goat myostatin through zygotes microinjection resulting in double-muscled phenotype in goats.
Bioscience reports - 21 Dec 2018
He Zhengyi, Zhang Ting, Jiang Lei, Zhou Minya, Wu Daijin, Mei Junyan, Cheng Yong
Abstract excerpt
Myostatin gene (MSTN) can inhibit the proliferation of myoblast, which in turn promotes muscle growth and inhibits adipocyte differentiation in livestock. MSTN mutation may lead to muscle hypertrophy or double-muscled (DM) phenotype. MSTN mutation animal, such as sheep, dog, and rabbit have been generated through CRISPR/Cas9 technology. However, goats with promising MSTN mutation have not been generated. We...
Topics
- Animals
- Animals, Genetically Modified
- Body Weight
- CRISPR-Cas Systems
- Gene Knockout Techniques
- Goats
- Microinjections
- Muscle, Skeletal
- Mutation
- Myostatin
- Phenotype