Article
Generation of myostatin edited horse embryos using CRISPR/Cas9 technology and somatic cell nuclear transfer.
Scientific reports - 24 Sept 2020
Moro Lucia Natalia, Viale Diego Luis, Bastón Juan Ignacio, Arnold Victoria, Suvá Mariana, Wiedenmann Elisabet, Olguín Martín, Miriuka Santiago, Vichera Gabriel
Abstract excerpt
The application of new technologies for gene editing in horses may allow the generation of improved sportive individuals. Here, we aimed to knock out the myostatin gene (MSTN), a negative regulator of muscle mass development, using CRISPR/Cas9 and to generate edited embryos for the first time in horses. We nucleofected horse fetal fibroblasts with 1, 2 or 5 µg of 2 different gRNA/Cas9 plasmids targeting the first...
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