Article
Efficient CRISPR/Cas9-mediated biallelic gene disruption and site-specific knockin after rapid selection of highly active sgRNAs in pigs.
Scientific reports - 21 Aug 2015
Wang Xianlong, Zhou Jinwei, Cao Chunwei, Huang Jiaojiao, Hai Tang, Wang Yanfang, Zheng Qiantao, Zhang Hongyong, Qin Guosong, Miao Xiangnan, Wang Hongmei, Cao Suizhong, Zhou Qi, Zhao Jianguo
Abstract excerpt
Genetic engineering in livestock was greatly enhanced by the emergence of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9), which can be programmed with a single-guide RNA (sgRNA) to generate site-specific DNA breaks. However, the uncertainties caused by wide variations in sgRNA activity impede the utility of this system in generating genetically modified pigs. Here,...
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