Article
Efficient Generation of Gene-Modified Pigs Harboring Precise Orthologous Human Mutation via CRISPR/Cas9-Induced Homology-Directed Repair in Zygotes.
Human mutation - 1 Jan 2016
Zhou Xiaoyang, Wang Lulu, Du Yinan, Xie Fei, Li Liang, Liu Yu, Liu Chuanhong, Wang Shiqiang, Zhang Shibing, Huang Xingxu, Wang Yong, Wei Hong
Abstract excerpt
Precise genetic mutation of model animals is highly valuable for functional investigation of human mutations. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9)-induced homology-directed repair (HDR) is usually used for precise genetic mutation, being limited by the relatively low efficiency compared with that of non-homologous end joining (NHEJ). Although inhibition of...
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