Efficient generation of gene-modified pigs via injection of zygote with Cas9/sgRNA.

doi:10.1038/srep08256

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Article

Efficient generation of gene-modified pigs via injection of zygote with Cas9/sgRNA.

Scientific reports - 5 Feb 2015

Wang Yong, Du Yinan, Shen Bin, Zhou Xiaoyang, Li Jian, Liu Yu, Wang Jianying, Zhou Jiankui, Hu Bian, Kang Nannan, Gao Jimin, Yu Liqing, Huang Xingxu, Wei Hong

Abstract excerpt

Co-injection of zygotes with Cas9 mRNA and sgRNA has been proven to be an efficient gene-editing strategy for genome modification of different species. Genetic engineering in pigs holds a great promise in biomedical research. By co-injection of one-cell stage embryos with Cas9 mRNA and Npc1l1 sgRNA, we achieved precise Npc1l1 targeting in Chinese Bama miniature pigs at the efficiency as high as 100%. Meanwhile,...

Topics

Alleles
Animals
Animals, Genetically Modified
Base Sequence
CRISPR-Cas Systems
Female
Gene Targeting
Membrane Proteins
Microinjections
Molecular Sequence Data
Mosaicism

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Organ Specificity

Ovary

RNA Editing

RNA, Messenger

Swine

Zygote

RNA, Guide, CRISPR-Cas Systems

Source

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Identifiers

PMID: 25653176
DOI: 10.1038/srep08256
PMCID: PMC4317696

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