Article
Production of gene-edited pigs harboring orthologous human mutations via double cutting by CRISPR/Cas9 with long single-stranded DNAs as homology-directed repair templates by zygote injection.
Transgenic research - 1 Dec 2020
Xie Fei, Zhou Xiaoyang, Lin Tingting, Wang Lulu, Liu Chuanhong, Luo Xi, Luo Lihua, Chen Huayu, Guo Kenan, Wei Hong, Wang Yong
Abstract excerpt
Precise gene editing of model organisms is required for accurately modeling human diseases and deciphering gene functions. In this study, we used a pair of guide RNAs (sgRNAs), which in vitro transcribed along with other CRISPR RNA components, to generate two cleavage sites flanking pig GJB2 (pGJB2) CDS. By using long single-stranded DNAs (lssDNA) as homology-directed repair (HDR) templates, we efficiently...
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