Article

CRISPR/Cas9-mediated integration of large transgene into pig potential safe harbor

2019-08-01

Abstract excerpt

<h4>ABSTRACT</h4> Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (Cas9) is a precise genome manipulating tool which can produce targeted gene mutations in various cells and organisms. Although CRISPR/Cas9 can efficiently generate gene knock-out, the gene knock-in efficiency mediated by homology-directed repair (HDR) remains low, especially for large fragment integration. In...

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Literature Corpus work: e0a3604a-bb3f-592d-9195-eed288520d16
DOI: 10.1101/722348

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