Article
Use of the CRISPR/Cas9 system to produce genetically engineered pigs from in vitro-derived oocytes and embryos.
Biology of reproduction - 1 Sept 2014
Whitworth Kristin M, Lee Kiho, Benne Joshua A, Beaton Benjamin P, Spate Lee D, Murphy Stephanie L, Samuel Melissa S, Mao Jiude, O'Gorman Chad, Walters Eric M, Murphy Clifton N, Driver John, Mileham Alan, McLaren David, Wells Kevin D, Prather Randall S
Abstract excerpt
Targeted modification of the pig genome can be challenging. Recent applications of the CRISPR/Cas9 system hold promise for improving the efficacy of genome editing. When a designed CRISPR/Cas9 system targeting CD163 or CD1D was introduced into somatic cells, it was highly efficient in inducing mutations. When these mutated cells were used with somatic cell nuclear transfer, offspring with these modifications were...
Topics
- Animals
- Animals, Genetically Modified
- Antigens, CD
- Antigens, CD1d
- Antigens, Differentiation, Myelomonocytic
- Blastocyst
- CRISPR-Cas Systems
- Cell Line
- Embryo Culture Techniques