Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Reporter Gene Silencing in Targeted Mouse Mutants Is Associated with Promoter CpG Island Methylation.

PloS one - 1 Jan 2015

Kirov Julia V, Adkisson Michael, Nava A J, Cipollone Andreana, Willis Brandon, Engelhard Eric K, Lloyd K C Kent, de Jong Pieter, West David B

Abstract excerpt

Targeted mutations in mouse disrupt local chromatin structure and may lead to unanticipated local effects. We evaluated targeted gene promoter silencing in a group of six mutants carrying the tm1a Knockout Mouse Project allele containing both a LacZ reporter gene driven by the native promoter and a neo selection cassette. Messenger RNA levels of the reporter gene and targeted gene were assessed by qRT-PCR, and...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to CpG Islands
Share to DNA Methylation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Gene Expression Profiling

Share to Gene Silencing

Share to Genes, Reporter

Share to Lac Operon

Share to Mice, Knockout

Share to Promoter Regions, Genetic

Source

Open in PubMed Open full text

Identifiers

PMID: 26275310
DOI: 10.1371/journal.pone.0134155
PMCID: PMC4537176

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Reporter Gene Silencing in Targeted Mouse Mutants Is Associated with Promoter CpG Island Methylation.PMID 26275310Read ↗

Transcriptome Analysis of Targeted Mouse Mutations Reveals the Topography of Local Changes in Gene Expression.Semantic proximity Read ↗

CpG content affects gene silencing in mice: evidence from novel transgenes.Semantic proximity Read ↗

Mouse DNA methyltransferase (MTase) deletion mutants that retain the catalytic domain display neither de novo nor maintenance methylation activity in vivo.Semantic proximity Read ↗

Distribution of spontaneous CpG-associated G:C --> A:T mutations in the lacZ gene of Muta mice: effects of CpG methylation, the sequence context of CpG sites, and severity of mutations on the activity of the lacZ gene product.Semantic proximity Read ↗

A transgenic mouse model ubiquitously overexpressing <i>Dnmt1s</i> mRNA lacks increased protein levelsSemantic proximity Read ↗

Distal transgene insertion affects CpG island maintenance during differentiation.Semantic proximity Read ↗

Transcriptional silencing and reactivation in transgenic zebrafish.Semantic proximity Read ↗

Imprinting by DNA methylation: from transgenes to endogenous gene sequences.Semantic proximity Read ↗

Reactivation of heritably silenced gene expression in mice.Semantic proximity Read ↗

The mechanism of somatic hypermutation studied with transgenic and transfected target genes.Semantic proximity Read ↗

Unexpected effects of a heterozygous dnmt1 null mutation on age-dependent DNA hypomethylation and autoimmunity.Semantic proximity Read ↗

Use of human placental alkaline phosphatase transgenes to detect somatic mutation in mice in situ.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.