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Article

Transcriptome Analysis of Targeted Mouse Mutations Reveals the Topography of Local Changes in Gene Expression.

PLoS genetics - 1 Feb 2016

West David B, Engelhard Eric K, Adkisson Michael, Nava A J, Kirov Julia V, Cipollone Andreanna, Willis Brandon, Rapp Jared, de Jong Pieter J, Lloyd Kent C

Abstract excerpt

The unintended consequences of gene targeting in mouse models have not been thoroughly studied and a more systematic analysis is needed to understand the frequency and characteristics of off-target effects. Using RNA-seq, we evaluated targeted and neighboring gene expression in tissues from 44 homozygous mutants compared with C57BL/6N control mice. Two allele types were evaluated: 15 targeted trap mutations...

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Identifiers

PMID: 26839965
DOI: 10.1371/journal.pgen.1005691
PMCID: PMC4739719

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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