Article
Mouse DNA methyltransferase (MTase) deletion mutants that retain the catalytic domain display neither de novo nor maintenance methylation activity in vivo.
Biological chemistry - 1 May 1997
Zimmermann C, Guhl E, Graessmann A
Abstract excerpt
The mammalian genome encodes a DNA cytosine-5-methyltransferase (MTase) of about 170 kDa that is apparently responsible for both de novo and maintenance methylation at CpG sites. Both methylation activities have to be regulated accurately to ensure correct developmental and cell type-specific gen...
Topics
- Animals
- Base Sequence
- Blotting, Northern
- Blotting, Southern
- Blotting, Western
- Catalysis
- Cellular Senescence
- DNA (Cytosine-5-)-Methyltransferases
- DNA Damage
- DNA Methylation
- Electrophoresis
- Fluorescent Antibody Technique