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Article

Genome Editing in Zebrafish by ScCas9 Recognizing NNG PAM.

Cells - 16 Aug 2021

Liu Yunxing, Liang Fang, Dong Zijiong, Li Song, Ye Jianmin, Qin Wei

Abstract excerpt

The CRISPR/Cas9 system has been widely used for gene editing in zebrafish. However, the required NGG protospacer adjacent motif (PAM) of Streptococcus pyogenes Cas9 (SpCas9) notably restricts the editable range of the zebrafish genome. Recently, Cas9 from S. canis (ScCas9), which has a more relaxed 5'-NNG-3' PAM, was reported to have activities in human cells and plants. However, the editing ability of ScCas9 has...

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Identifiers

PMID: 34440868
DOI: 10.3390/cells10082099
PMCID: PMC8392876

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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