Article

A Cas9 with Complete PAM Recognition for Adenine Dinucleotides

2018-09-27

Abstract excerpt

CRISPR-associated (Cas) DNA-endonucleases are remarkably effective tools for genome engineering, but have limited target ranges due to their protospacer adjacent motif (PAM) requirements. We demonstrate a critical expansion of the targetable sequence space for a Type-IIA CRISPR-associated enzyme through identification of the natural 5’-NAA-3’ PAM specificity of a Streptococcus macacae Cas9 (Smac Cas9). We further...

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Literature Corpus work: 645b5581-51fe-59fa-af4a-cdcd6b097f9a
DOI: 10.1101/429654

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