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Exploring the protein stability landscape: Bacillus subtilis lipase A as a model for detergent tolerance.

Chembiochem : a European journal of chemical biology - 13 Apr 2015

Fulton Alexander, Frauenkron-Machedjou Victorine Josiane, Skoczinski Pia, Wilhelm Susanne, Zhu Leilei, Schwaneberg Ulrich, Jaeger Karl-Erich

Abstract excerpt

A systematic study was conducted with Bacillus subtilis lipase A (BSLA) to determine the effect of every single amino acid substitution on detergent tolerance. BSLA is a minimal α/β-hydrolase of 181 amino acids with a known crystal structure. It can be expressed in Escherichia coli and is biochemically well characterized. Site saturation mutagenesis resulted in a library of 3439 variants, each with a single amino...

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PMID: 25773356
DOI: 10.1002/cbic.201402664

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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