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Article

Insights of conformational dynamics on catalytic activity in the computational stability design of Bacillus subtilis LipA.

Archives of biochemistry and biophysics - 15 Jun 2022

Dong Fangying, Zhang Meng, Ma Rui, Lu Cheng, Xu Fei

Abstract excerpt

In protein engineering, the contributions of individual mutations to designed combinatorial mutants are unpredictable. Screening designed mutations that affect enzyme catalytic activity enables evolutions towards efficient activities. Here, Bacillus subtilis LipA (BSLA) was selected as a model protein for thermostabilization designs, and the circular dichroism measurements showed six combinatorial designs with...

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Identifiers

PMID: 35339426
DOI: 10.1016/j.abb.2022.109196

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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