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Article

Improving the catalytic activity and stability of Bacillus alcalophilus serine protease BAPB92 by rational design.

International journal of biological macromolecules - 1 Dec 2024

Yuan Yuan, Wei Tingting, Qiang Jiyu, Wen Yangxuan, Lu Jing, Shi Yawei, Zhang Jian

Abstract excerpt

The catalytic activity and stability of proteases are essential for their application in the detergent industry. To enhance the catalytic properties of BAPB92, homologous sequence comparison combined with rational design was employed. Six mutants were generated: BAPB92 (A188P), BAPB92 (V262I), BAPB92 (Q239R), BAPB92 (A188P/V262I), BAPB92 (Q239R/V262I), and BAPB92 (Q239R/A188P). Remarkably, the mutant BAPB92...

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PMID: 39557243
DOI: 10.1016/j.ijbiomac.2024.137782

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