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Article

Engineering the loops in a lipase for stability in DMSO.

Protein engineering, design & selection : PEDS - 1 Apr 2013

Yedavalli Poornima, Rao Nalam Madhusudhana

Abstract excerpt

Nearly 65% of the surface of a lipase, from Bacillus subtilis, is occupied by the loops. Since the loops are dynamic components of a protein, located on the surface and are tolerant to substitutions, we subjected all 91 amino acids of the loops to site saturation mutagenesis to identify mutations that improve the stability and activity of lipase in dimethyl sulfoxide (DMSO). Based on a novel screening system, we...

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Identifiers

PMID: 23404771
DOI: 10.1093/protein/gzt002

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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