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Article

In vitro evolved non-aggregating and thermostable lipase: structural and thermodynamic investigation.

Journal of molecular biology - 28 Oct 2011

Kamal Md Zahid, Ahmad Shoeb, Molugu Trivikram Rao, Vijayalakshmi Amash, Deshmukh Mandar V, Sankaranarayanan Rajan, Rao Nalam Madhusudhana

Abstract excerpt

Rational and in vitro evolutionary approaches to improve either protein stability or aggregation resistance were successful, but empirical rules for simultaneous improvement of both stability and aggregation resistance under denaturing conditions are still to be ascertained. We have created a robust variant of a lipase from Bacillus subtilis named "6B" using multiple rounds of in vitro evolution. T(m) and optimum...

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PMID: 21925508
DOI: 10.1016/j.jmb.2011.09.002

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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