Article
Mutatomics analysis of the systematic thermostability profile of Bacillus subtilis lipase A.
Journal of molecular modeling - 1 Jun 2014
Tian Feifei, Yang Cao, Wang Congcong, Guo Tailin, Zhou Peng
Abstract excerpt
Use of point mutagenesis technique to improve protein thermostability is a routine strategy in the protein engineering community and directed evolution approach has been widely utilized to fulfill this. However, directed evolution often does not assure a minimalist design for obtaining a desired property in proteins, and other traditional methods such as error-prone PCR and iterative saturation mutagenesis are...
Topics
- Bacillus subtilis
- Bacterial Proteins
- Enzyme Stability
- Genotype
- Least-Squares Analysis
- Linear Models
- Models, Chemical
- Molecular Dynamics Simulation
- Mutagenesis, Site-Directed
- Nonlinear Dynamics
- Phenotype