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Article

Surfactant-tolerance evolution of Bacillus clausii protease for enhancing activity and stability by reshaping the substrate access tunnel.

Colloids and surfaces. B, Biointerfaces - 1 Jan 2025

Ma Xiangyang, Wang Liya, Chen Jingyu, Guo Enping, Zheng Hongchen, Zhao Lei, Lu Fuping, Liu Yihan

Abstract excerpt

Alkali proteases are crucial in numerous industries, especially in the laundry industry, but their inactivation by surfactants limits their effectiveness. This study employed substrate access tunnel engineering to improve the performance of WT bcPRO in surfactants. By modifying the key residues in the substrate pocket, the best variant N212S showed higher stability and activity in both AES and LAS. Molecular...

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PMID: 39366107
DOI: 10.1016/j.colsurfb.2024.114289

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