The AXXXA motif: A rational design strategy and experimental studies to enhance both activity and thermostability of BTL2 lipase.

doi:10.1016/j.enzmictec.2026.110884

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Article

The AXXXA motif: A rational design strategy and experimental studies to enhance both activity and thermostability of BTL2 lipase.

Enzyme and microbial technology - 1 Aug 2026

Ghorban Mahsa, Yakhchali Bagher, Rezaei Mehran Habibi, Aminzadeh Saeed, Haghbeen Kamahldin, Karkhanei Behrouz, Karkhane Ali Asghar

Abstract excerpt

Thermostability is a critical limitation for industrial lipases. Rational design using stabilizing structural motifs like AXXXA offers a targeted engineering approach. This study aimed to enhance both thermostability and catalytic efficiency of Bacillus thermocatenulatus lipase (BTL2) through com...

Topics

Lipase
Enzyme Stability
Bacterial Proteins
Kinetics
Bacillus
Amino Acid Motifs
Mutagenesis, Site-Directed
Protein Engineering
Catalytic Domain
Models, Molecular
Hydrogen-Ion Concentration
Mutation
Temperature
Substrate Specificity
Geobacillus

Source

Open in PubMed

Identifiers

PMID: 42085918
DOI: 10.1016/j.enzmictec.2026.110884

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-42085918-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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