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Article

Impact of three Illumina library construction methods on GC bias and HLA genotype calling.

Human immunology - 1 Mar 2015

Lan James H, Yin Yuxin, Reed Elaine F, Moua Kevin, Thomas Kimberly, Zhang Qiuheng

Abstract excerpt

Next-generation sequencing (NGS) is increasingly recognized for its ability to overcome allele ambiguity and deliver high-resolution typing in the HLA system. Using this technology, non-uniform read distribution can impede the reliability of variant detection, which renders high-confidence genoty...

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Identifiers

PMID: 25543015
DOI: 10.1016/j.humimm.2014.12.016
PMCID: PMC5089167

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Prepared record publication-25543015-2026-09-05.1. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 5 Sept 2026.

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