Article
Ultra-high resolution HLA genotyping and allele discovery by highly multiplexed cDNA amplicon pyrosequencing.
BMC genomics - 6 Aug 2012
Lank Simon M, Golbach Brittney A, Creager Hannah M, Wiseman Roger W, Keskin Derin B, Reinherz Ellis L, Brusic Vladimir, O'Connor David H
Abstract excerpt
BACKGROUND: High-resolution HLA genotyping is a critical diagnostic and research assay. Current methods rarely achieve unambiguous high-resolution typing without making population-specific frequency inferences due to a lack of locus coverage and difficulty in exon-phase matching. Achieving high-r...
Topics
- Alleles
- Base Sequence
- Cell Line
- DNA Primers
- DNA, Complementary
- Genes, MHC Class I
- Genes, MHC Class II
- Genotype
- Genotyping Techniques
- HLA Antigens
- High-Throughput Nucleotide Sequencing