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Article

Bias from removing read duplication in ultra-deep sequencing experiments.

Bioinformatics (Oxford, England) - 15 Apr 2014

Zhou Wanding, Chen Tenghui, Zhao Hao, Eterovic Agda Karina, Meric-Bernstam Funda, Mills Gordon B, Chen Ken

Abstract excerpt

MOTIVATION: Identifying subclonal mutations and their implications requires accurate estimation of mutant allele fractions from possibly duplicated sequencing reads. Removing duplicate reads assumes that polymerase chain reaction amplification from library constructions is the primary source. The alternative-sampling coincidence from DNA fragmentation-has not been systematically investigated. RESULTS: With...

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Identifiers

PMID: 24389657
DOI: 10.1093/bioinformatics/btt771
PMCID: PMC3982159

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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