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Article

Report on the effects of fragment size, indexing, and read length on HLA sequencing on the Illumina MiSeq.

Human immunology - 1 Dec 2015

Profaizer T, Coonrod E M, Delgado J C, Kumánovics A

Abstract excerpt

Single-molecule sequencing should allow for unambiguous, accurate, and high-throughput HLA typing. In this proof of principle study, we investigated the effects of fragment size for library preparation, indexing strategy, and read length on HLA typing. Whole gene amplicons of HLA-A, B, C, DRB1, and DQB1 were obtained by long-range PCR. For library preparation, two fragment sizes were evaluated: 100-300bp and...

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PMID: 26303189
DOI: 10.1016/j.humimm.2015.08.002

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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