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Article

Impact of Reducing DNA Input on Next-Generation Sequencing Library Complexity and Variant Detection.

The Journal of molecular diagnostics : JMD - 1 May 2020

McNulty Samantha N, Mann Patrick R, Robinson Joshua A, Duncavage Eric J, Pfeifer John D

Abstract excerpt

PCR amplification, a key step in next-generation sequencing (NGS) library construction, can generate an unlimited amount of product from limited input; however, it cannot create more information than was present in the original template. Thus, NGS libraries can be made from very little DNA, but reducing the input may compromise assay sensitivity in ways that are difficult to ascertain unless library complexity...

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PMID: 32142899
DOI: 10.1016/j.jmoldx.2020.02.003

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