Article

Optimization of Enzymatic Fragmentation is Crucial To Maximize Genome Coverage: A Comparison of Library Preparation Methods for Illumina Sequencing

2021-10-21

Abstract excerpt

<h4>Background: </h4> Novel commercial kits for whole genome library preparation for next-generation sequencing on Illumina platforms promise shorter workflows, lower inputs and cost savings. Time savings are achieved by employing enzymatic DNA fragmentation and by combining end-repair and tailing reactions. Fewer cleanup steps also allow greater DNA input flexibility (1 ng-1 µg), PCR-free options from 100 ng DNA,...

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Literature Corpus work: d104a81c-a011-5ff5-97ab-01a335f97c29
DOI: 10.21203/rs.3.rs-965847/v1

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